Tsh rfx on abnormal to free t4 labcorp. Both of my parents and my sister have issues with theirs. My TSH is 3.52, lab says standard range is 0.27 - 4.20. The T4 Free is 1.0 and the lab range is 0.9 - 1.8. My current doctor suggests this is not a problem. I have done to much reading and can't get a grasp on what is "normal".

TSH normal range. According to the U.S. National Library of Medicine the normal TSH range in healthy adults is 0.4 to 4.0 milli-international units per liter (0.4 – 4.0 mIU/L). 7 For your thyroid to be working correctly, your lab test results for normal free T4 levels should be 9 – 23 pmol/L (0.7 – 1.8 ng/dL)

Tsh rfx on abnormal to free t4 labcorp. There is good correlation of free T4 by equilibrium dialysis and free T4 by liquid chromatography-tandem mass spectrometry (LC-MS/MS). 48 In a comparison of the IA and LC-MS/MS method in 109 patients, the correlation between free T4 and TSH was quite poor for the IA method and substantially better for LC-MS/MS. 49 Indeed, when the TSH was …

I had my thyroid ablated 27 years ago for Grave's disease. My thyroid has been relatively stable over the years with thyroid supplements, but occasional changes in dosage have been needed. This year Medicare is denying payment on having my thyroid levels (tsh and free T4) checked. It has been a little over a year since they were last checked.

Thyroid Testing Including TSH National Coverage Determination. CPT Codes: Code Description 84436 Thyroxine; total 84439 Thyroxine; free 84443 Thyroid stimulating hormone (TSH) 84479 Thyroid hormone (T3 or T4) uptake or thyroid hormone binding ratio (THBR) Code Description A18.81 Tuberculosis of thyroid gland Dr. Philip Kern answered. Endocrinology 45 years experience. Thyroid replacement: The TSH increases when the ft4 is too low. But the relationship is not precise. You likely need a higher dose of levothyroxine. See your doctor. Created for people with ongoing healthcare needs but benefits everyone.

If you have a low level of TSH, it may mean that your thyroid is producing too much T3 or T4. Thyroxine (T4) T4 is one of the hormones that is produced by your thyroid. It helps to regulate things like your energy level, mood, and body temperature. Sometimes, T4 measurements are divided into two categories: total T4 and free T4.If your TSH level is elevated to between 5.5 and 10 mIU/L and your T4 is still in the normal range, you and your doctor will consider starting thyroid medication or repeating the TSH later on. If you have symptoms of hypothyroidism or you have a positive test for anti-thyroid antibodies, you might want to start thyroid pills right away.Thyroid-stimulating hormone (TSH); free thyroxine, direct Special Instructions This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R).MEDICAL PROFESSIONAL. Mark Lupo, M.D. The reason for the MRI is to test for the rare entity of a TSH secreting pituitary tumor. I would first retest the TSH, free T4 and free T3 -- if still elevated, proceed with MRI. there is also a perhaps more rare entity called thyroid hormone resistance which can cause TSH, T4 and T3 to all be elevated.Purchase a TSH Test today on MDsave. Costs range from $8 to $180. Those on high deductible health plans or without insurance can save when they buy their procedure upfront through MDsave. Read more about how MDsave works. Search board-certified providers, compare prices, buy online, and save money.If the second test comes back abnormal again, I'd suggest looking at 259.9. Remember a oder cannot render a dx so you have to look at what the provider states. We cannot code based on the lab result. If theprovider does not document abnormal result then we cannot code even that. And we cannot code an endocrine disorder based on an abnormal result.What your Free Thyroid Hormones Mean for You (Free T3 & Free T4) Most physicians (Doctors) tend to order what is known as a TSH with reflex to free T4.. This lab test is supposed to only order the free T4 IF the TSH is abnormal (2).. The idea is that it saves on the cost of unnecessary lab tests because why would you need to see the free T4 if the TSH is normal?• Free thyroxine (T4) and free triiodothyronine (T3) assays by dialysis and HPLC/MS-MS, which provides enhanced accuracy over commonly used analog (automated) methods.7-10 Dialysis and HPLC/MS-MS may be preferred for evaluating patients who have conditions that impact protein binding capacity, including those who:

Total T4 (TT4) measurement is preferred to free T4 (FT4) especially in last part of pregnancy. Guidelines by American Thyroid Association, European Thyroid Association and Endocrine Society state that TT4 increases 1.5 times pre-pregnant levels after week 16 of pregnancy. However, this is based on a small study conducted 40 years ago which used radioimmunoassay for determination in changes in TT4.A common cause of elevated T 4 in nonthyroidal disease is said to be liver disease. Serum thyroxine and free thyroxine index (FTI) are increased in familial dysalbuminemic hyperthyroxinemia, a euthyroid syndrome in which an abnormal binding site has affinity for thyroxine. 4 The T 3 is usually normal in this entity, as is T 3 uptake (THBR ...TSH Rfx on abnormal to Free T4 - 1.210 uIU/mL range 0.450 - 4.500. DHEA-S - 351 ug/dL range 280 - 640. Free testosterone, direct - 18.2 pg/mL range 9.3 - 26.5. If anyone has any comments or concerns PLEASE FEEL FREE to drop a line… I would really appreciate ANY FEEDBACK that would help me reach normal blood levels… thanks.

Hypothyroidism with normal TSH levels can still lead to common symptoms of low thyroid function. These symptoms can include: Chronic fatigue. Weight gain despite no change in diet. Slow heart rate. Shortness of breath with exertion (exercise) Feeling cold all the time. Muscle and joint aches. Constipation.

What is subclinical hyperthyroidism? Subclinical hyperthyroidism happens when you have a low or undetectable thyroid-stimulating hormone (TSH) level with normal thyroxine (T4) and triiodothyronine (T3) levels.. You don’t technically have hyperthyroidism (commonly called overt hyperthyroidism, in comparison), but it has the potential to develop into overt …

The thyroid is a butterfly-shaped gland in the front of the neck. Thyroid function is controlled by the pituitary gland, which produces TSH and releases it into the bloodstream. TSH stimulates the thyroid gland to produce thyroid hormones (T3 and T4). TSH will also tell the body to make or release thyroid hormones.TSH then stimulates cells in your thyroid to release thyroxine or T4 (80%) and triiodothyronine or T3 (20%) into your bloodstream. These two hormones prevent your pituitary gland from producing more TSH if the levels of thyroxine and triiodothyronine are too high, thus completing the cycle. When T4 and T3 levels drop, the cycle starts over …Dr. Quang Nguyen answered. Specializes in Endocrinology. Normal: That's a normal value. That's why they didn't have to run the free t4. Reflex means they will run ft4 only if tsh is abnormal. Created for people with ongoing healthcare needs but benefits everyone. Learn how we can help.This assay uses a competition principle. 15 μL of sample is incubated with a T4-specific antibody labeled with a ruthenium complex; bound T4 is released from binding proteins in the sample by ANS. After addition of streptavidin-coated microparticles and biotinylated T4, the still-free binding sites of the labeled antibody become occupied, with

Thyroxine (T4), Free, Direct (Free T4) LabCorp. SKU. 349-l. LabCorp Test #: 001974, CPT: 84439. Details. The thyroid gland produces and secretes T4, otherwise known as thyroxine. Proteins bind to T4 and carry it throughout the bloodstream. Once in the tissues, T4 is released from the proteins and is now free to convert into the more active form ...Article Reflex TSH strategy: the good, the bad and the ugly was published on January 1, 2020 in the journal Clinical Chemistry and Laboratory Medicine (CCLM) (volume 58, issue 1). ... Walsh JP. Rationalizing thyroid function testing: which TSH cutoffs are optimal for testing free T4? J Clin Endocrinol Metab 2017;102:4235-41. 10.1210/jc.2017 ...Dr. Alan Feldman answered. 43 years experience. Mildly abnormal: That TSH is mildly high, implying that you may have mild hypothyroidism. The free T4 is likely to be in the lower range of normal. Most people with ... Read More. Created for people with ongoing healthcare needs but benefits everyone.It also continuously monitors the level of thyroid hormones present in your bloodstream. 1 If it determines that energy levels are low, it sends out TRH, Thyroid Releasing Hormone, to your pituitary gland. Your pituitary gland then releases TSH, or Thyroid Stimulating Hormone. TSH is sent directly to your thyroid to produce more T4, …presented in Table-3. Higher level of free T4 in men than women. For men the range was 0.80-1.72 and for women 0.80-1.70 (p<0.001). There was no increase in free T4 with age. The 95% reference interval for free T4 was 0.8-1.70 ng/dL, while for kit manufacturer it was 0.93-1.70 ng/dL. The total T4 reference values in μg/dL (2.5thSubclinical hypothyroidism happens when you have elevated thyroid-stimulating hormone (TSH) levels with normal levels of thyroxine (T4). You don't technically have hypothyroidism (commonly called overt hypothyroidism, in comparison), but it has the potential to develop into overt hypothyroidism. Hypothyroidism happens when your thyroid doesn ...If your TSH level is elevated to between 5.5 and 10 mIU/L and your T4 is still in the normal range, you and your doctor will consider starting thyroid medication or repeating the TSH later on. If you have symptoms of hypothyroidism or you have a positive test for anti-thyroid antibodies, you might want to start thyroid pills right away.Dry skin. Feeling “down” or “depressed”. Mild brain fog or clouded thinking/judgment. The degree of symptoms that you will experience will depend on the degree of elevation of your TSH (5). Put simply: The higher your TSH, the more your thyroid gland is compromised and the more severe your symptoms will be.Therefore, total T4 should not be used as the only marker for thyroid function evaluation. Thyrotropin (TSH) may be better than T4 as the initial test of thyroid status. TSH is elevated in primary hypothyroidism. TSH is low in primary hyperthyroidism. Free T4 may more accurately measure the physiologic amount of T4.Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC; 322000: Comp. Metabolic Panel (14) 24323-8: 001032: Glucose: mg/dL: 2345-7T4 (thyroxine) is the main hormone produced by the thyroid gland. A laboratory test can be done to measure the amount of free T4 in your blood. Free T4 is the thyroxine that is not attached to a protein in the blood. Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand.• Obtaining a T3 Uptake or Free T4 Index is not usually helpful. Instead it should be a Total T3 level or Free T4. • Children with Trisomy 21 often have mildly elevated TSH levels (hyperthyrotropenemia) with normal Total T4 or Free T4. Generally referral is not needed unless there are positive thyroid antibodies, or rising TSH. Call with ...Automated free T4 methods typically fall into one of two categories - assays in which a T4 analog carries the detector label and assays in which an anti-T4 antibody carries the detector label. ... If TSH is abnormal, free T4 should be measured to confirm disease and identify the underlying mechanism. Autoantibody measurement is a useful ...Hypothyroidism with normal TSH levels can still lead to common symptoms of low thyroid function. These symptoms can include: Chronic fatigue. Weight gain despite no change in diet. Slow heart rate. Shortness of breath with exertion (exercise) Feeling cold all the time. Muscle and joint aches. Constipation.Measurement of total rather than free T4 can be useful in situations where there is a suspicion of abnormal T4 binding proteins. For example, total T4 will be elevated in the presence of anti-T4 antibodies and in FDH. ... FDH or TFT antibody interference can give elevated fT4 results with a TSH within the reference interval. Attempts by the ...The supplemental measurement of free T4 in patients with abnormal TSH measurements allows one to better assess the severity of the changes. Serum T3 (triiodothyronine) levels often are depressed in sick and hospitalized patients, caused in part by the biochemical shift to the production of reverse T3. Therefore, T3 generally is not a reliable ...Dry skin. Feeling “down” or “depressed”. Mild brain fog or clouded thinking/judgment. The degree of symptoms that you will experience will depend on the degree of elevation of your TSH (5). Put simply: The higher your TSH, the more your thyroid gland is compromised and the more severe your symptoms will be.The normal range for thyroid-stimulating hormone (TSH) levels in adults typically falls between 0.5-4 mU/L . In addition to TSH, the concentration of free thyroxine (tsh w/reflex to ft4) and total thyroxine (TT4) can also provide valuable information about thyroid function. For a healthy, average person without any conditions that cause ... This test uses a T4 analog, rather than equilibrium dialysis, to determine the amount of free T4. It therefore differs from the T4 Free, Direct Dialysis test (test code 35167). In the general population, the analog FT4 test is most useful as a follow-up test when TSH results are abnormal.

Nov 11, 2022 · Free T4 tests are commonly ordered after a TSH blood test is found to be abnormal. Elevated Free T4 results may indicate hyperthyroidism. Low Free T4 results may indicate hypothyroidism. LabCorp Example. Free T4 reference range: 0.82 to 1.77 ng/dL. Quest Diagnostics Example. Free T4 reference range: 0.8 to 1.8 ng/dL. Thyroglobulin Antibody Test ... Examples for TSH low T4 normal: 1. Female non pregnant, 35 years old results: Tsh less than 0.1 (<0.1 mU/l), Normal TSH level for adults is 0.3 - 4.5 mIU/ml, the result is lower than normal range. FT3 2.5, normal FT3 for adults is 1.5 - 4,2 pg/ml, normal result. FT4 1.2, normal FT4 for adults is 0.9 - 1.9 ng/dl, normal result.What is a normal TSH with reflex to FT4? TSH normal values are 0.5 to 5.0 mIU/L. Pregnancy, a history of thyroid cancer, history of pituitary gland disease, and older age are some situations when TSH is optimally maintained in different range as guided by an endocrinologist. FT4 normal values are 0.7 to 1.9ng/dL.TSH Rfx on Abnormal to Free T4 TSH 1.700 0.450-4.500 uIU/mL Final results 01 ... You need to request some further tests such as free t3 & t4, reverse t3 and also ...TSH then stimulates cells in your thyroid to release thyroxine or T4 (80%) and triiodothyronine or T3 (20%) into your bloodstream. These two hormones prevent your pituitary gland from producing more TSH if the levels of thyroxine and triiodothyronine are too high, thus completing the cycle. When T4 and T3 levels drop, the cycle starts over again. Prolactin Test Current Result and Flag Previous Result and Date Units Reference Interval Prolactin 01 5.0 ng/mL 4.8-23.3 TSH Rfx on Abnormal to Free T4 Test Current Result and Flag Previous Result and Date Units Reference Interval TSH 01 0.858 uIU/mL 0.450-4.500 Estrogens, Total Test Current Result and Flag Previous Result and Date Units ...In cases with marginally elevated TSH (6 to 10 mIU/L), monitor carefully, repeating a serum TSH and free T4 in a week. However, if the serum TSH does not normalize by four to six weeks of age, thyroxine treatment is recommended. Low or normal TSH and low free T4 concentrations suggest central hypothyroidism. As TSH is not elevated, this form of ...Free T3 - T3 is the most powerful and potent thyroid hormone in your body. Most of it is created when your body turns T4 into T3. Reverse T3 - Reverse T3 is an anti-thyroid metabolite that acts to completely BLOCK your thyroid from working. Reverse T3 is created by the body from T4. Your body can either turn T4 into T3 or into reverse T3 ...

If there is not enough T3 and T4 in the blood, your pituitary gland will increase levels of TSH as a result. Elevated TSH levels usually cause symptoms of hypothyroidism like fatigue, constipation, joint pain, a puffy face, or heavy menstrual bleeding (in women). The reasons for levels of TSH being higher than the normal range can include ...From there you can check your lab tests every 6-8 weeks to follow your TSH, free T3, free t4, and reverse T3 . Dosing varies quite a bit based on the person but a typical dose may range between 25mcg and 250mcg of Levothyroxine or Synthroid per day. If you have continued to increase your dose beyond 150mcg and haven't noticed ANY improvement ...Screening for thyroid dysfunction using TSH and free T4 levels is recommended before treatment initiation, at 4-6 weekly intervals, and should be repeated before each treatment cycle. For confirmed primary and central hypothyroidism, levothyroxine therapy is started, but hypocortisolism should be ruled out prior to treating central hypothyroidism.The test, called a thyroid function test, looks at levels of thyroid-stimulating hormone (TSH) and thyroxine (T4) in the blood. Doctors may refer to this as "free" T4 (FT4). A high level of TSH and a low level of T4 in the blood could mean you have an underactive thyroid. If your test results show raised TSH but normal T4, you may be at risk of ...Normal levels of free T4 range from 0.8-1.8 nanograms per deciliter (ng/dl) of blood. Normal T4 levels also vary across trimesters of pregnancy. A 2011 study in the Journal of Thyroid Research ...FSH, Serum quantity. Add to cart. SKU: 004309 Category: LabQuestTSH: Detects TSH levels for differential diagnosis of primary, secondary, and tertiary hypothyroidism; also useful in screening for hyperthyroidism: 867: T4 (Thyroxine), Total: Used to help diagnose hypothyroidism and hyperthyroidism: 861: T3 Uptake: Used with measurement of thyroxine (T4) to calculate the free T4 index to assess thyroid ...I've been on 100mcg of Synthroid since May 2018. I was diagnosed with Hashimoto's in January 2017 with a TSH of 31. As a disclaimer, I am treated by…The normal range for thyroid-stimulating hormone (TSH) levels in adults typically falls between 0.5-4 mU/L . In addition to TSH, the concentration of free thyroxine (tsh w/reflex to ft4) and total thyroxine (TT4) can also provide valuable information about thyroid function. For a healthy, average person without any conditions that cause ...A free T4 level in the optimal range usually indicates normal thyroid gland function. A free T4 level in the normal range is usually accompanied by a TSH level that is also in the normal range. When combined with an elevated TSH level, a normal free T4 may indicate subclinical hypothyroidism. When combined with a low TSH level, a normal free T4TSH remains the preferred test during pregnancy and in situations affecting thyroid hormone binding proteins. A serum TSH above the upper reference limit with a normal free T4 level indicates subclinical hypothyroidism, which has a prevalence of 4.3–8.5%. 3 Mild TSH fluctuations may occur in patients without thyroid disease, with …Normal Reference Range for Serum TSH, Free T4, T otal T4, and T otal T3 on Roche® Platforms in . Basrah, Iraq . Nassar Taha Alibrahim 1, Samih Abed Odhaib 2, Ali Hussain Alhamza 1,tests results. When a test for total thyroxine (total T4 or T4 radioimmunoassay) or T3 uptake is performed, calculation of the free thyroxine index (FTI) is useful to correct for abnormal results for either total T4 or T3 uptake due to protein binding effects. Indications: Thyroid function tests are used to define hyper function, euthyroidism, orWhat is normal range for TSH and free T4? TSH normal values are 0.5 to 5.0 mIU/L. Pregnancy, a history of thyroid cancer, history of pituitary gland disease, and older age are some situations when TSH is optimally maintained in different range as guided by an endocrinologist. FT4 normal values are 0.7 to 1.9ng/dL. What is TSH W Free T4 RFX …A normal level of Free T4 ranges between 0.8 to 1.5 micrograms per deciliter (ng/dL). It can vary depending on the laboratory assay used. Total T4 is not normally done except in pregnancy when it is more accurate than Free T4 test. A normal Total T4 range is 5-12 ug/dl. Low levels of T4 are seen with hypothyroidism, while high levels are seen ...We would like to show you a description here but the site won't allow us.RPR. RPR titer. Treponemal Ab (TPAb) Interpretation. Nonreactive. Not performed. Not performed. No laboratory evidence of syphilis. If recent exposure is suspected, submit a new sample for testing in 2-4 weeks.I've been on 100mcg of Synthroid since May 2018. I was diagnosed with Hashimoto's in January 2017 with a TSH of 31. As a disclaimer, I am treated by…

Blood test says TSH rfx on abnormal to free T4 -349829 and TSH 0.035, T4 free (direct) 1.26 what does this mean ? 2 doctor answers • 2 doctors weighed in Last updated Apr 20, 2019

Thyroxine, also known as T4, is a type of thyroid hormone. A T4 test measures the level of T4 in your blood. Too much or too little T4 can be a sign of thyroid disease. There are two forms of T4 in your blood: Free T4 is the active form of thyroxine hormone that enters your tissues where it's needed. Bound T4 is thyroxine that attaches or binds ...

The Free T4 test is used to assess thyroid function, indicate hypothyroidism or hyperthyroidism, help diagnose thyroid disease, and evaluate the effectiveness of thyroid treatment. Thyroxine (T4) is one of the two main hormones produced by the thyroid, a butterfly-shaped gland in your throat. Along with the hormone Triiodothyronine (T3), T4 ...FTI is a physiologic index of metabolic activity that correlates with free thyroxine (T 4) Special Instructions This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R).Normal TSH: Tsh rfx means that if the TSH level was abnormal, the lab would then automatically check your thyroid level with a free t4.Since your TSH was normal, the free T4 was not done. The glucose of 56 is low. Are you on meds that can lower your sugar? Sometimes the lab test will be inaccurate if the blood has been sitting for a long time before it was processed.Test Result Name. Result LOINC Value. Applies only to results expressed in units of measure originally reported by the performing laboratory. These values do not apply to results that are converted to other units of measure. FRT4. T4 (Thyroxine), Free, S. 83122-2. Evaluating suspected thyroid function disorders using free thyroxine measured ...Thyroid-stimulating hormone (TSH); free thyroxine, direct Special Instructions This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R).Thyroid activity is controlled by the hypothalamus, which produces thyrotropin-releasing hormone to tell the pituitary gland to make TSH, thereby causing the thyroid to produce more T4 and T3 when levels are low.If T4 and T3 levels are high, the hypothalamus reduces TRH production and thus, less TSH is made. This process helps keep T4 and T3 in balance [].A low level of TSH and high levels of T3 and/or T4 usually means you have an overactive thyroid. Doctors may refer to these measurements as "free" T3 and T4 (FT3 and FT4). What's considered normal varies depending on things such as your age and the exact testing technique used by the laboratory. Find out more about thyroid function tests on …Introduction. We describe changes in serum free thyroxine (fT4) and free tri‐iodothyronine (fT3) with normal levels of TSH during heparin treatment. fT4 and fT3 concentrations may rise markedly after both fractionated and unfractionated heparin, independently of the route of administration. 1, 2 However, the changes are an artefact, related to displacement of thyroid hormones from their ...

1522 east southern avenuemybenefitresourcesshooting in springfield va today30 day weather forecast lansing mi Tsh rfx on abnormal to free t4 labcorp touchpaydirect.com login [email protected] & Mobile Support 1-888-750-8309 Domestic Sales 1-800-221-2545 International Sales 1-800-241-4583 Packages 1-800-800-5546 Representatives 1-800-323-4424 Assistance 1-404-209-5108. these situations, it is better to ask both for TSH and free T4 for thyroid evaluation, which will typically be in the normal range. • Biotin, a commonly taken over-the-counter supplement, can cause the measurement of several thyroid function tests to appear abnormal, when they are in fact normal in the blood.. rs3 royal crossbow Dry skin. Feeling “down” or “depressed”. Mild brain fog or clouded thinking/judgment. The degree of symptoms that you will experience will depend on the degree of elevation of your TSH (5). Put simply: The higher your TSH, the more your thyroid gland is compromised and the more severe your symptoms will be.TSH - TSH tests pituitary function and can be used to diagnose thyroid disease. Free T3 - Free T3 may be the most important measure of thyroid function in the serum because it measures the free and active thyroid hormone. T3 is more biologically active than T4. Free T4 - Free T4 measures the amount of free T4 in circulation. In order to be ... zoar valley motorsportscancelling threadbeast FTI is a physiologic index of metabolic activity that correlates with free thyroxine (T 4) Special Instructions This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). asknow tteccostco gas prices merrillville New Customers Can Take an Extra 30% off. There are a wide variety of options. Normal Reference Range for Serum TSH, Free T4, T otal T4, and T otal T3 on Roche® Platforms in . Basrah, Iraq . Nassar Taha Alibrahim 1, Samih Abed Odhaib 2, Ali Hussain Alhamza 1,Thyroid function tests (TFTs) The term ' thyroid function tests ' refers to the following investigations: TSH (0.4 - 4 mU/L) Free T4 (9 - 25 pmol/L) Free T3 (3.5 - 7.8 nmol/L) There are separate reference ranges for children and pregnant women. Reference ranges for TFTs often vary between labs, so always refer to your local guidelines.The Access Free T4 assay is a -twostep binding enzyme immunoassay. Monoclonal anti-Thyroxine (T4) antibody coupled to biotin, sample, buffered protein solution, and streptavidin-coated solid phase are added to the reaction vessel. The anti-T4 antibody coupled to biotin binds to the solid phase and the free T4 in the sample.